A penny for your method: AMPure substitute

NOTE:  This is a repost of an entry that I wrote for the molecularecologist.com. This week may stand as the most exciting week of the year for decreasing the cost of PCR cleanup, DNA cleanup, and library prep. Why, you ask? Because a great paper was published over in…

A penny for your method: Nutator

NOTE:  This is a repost of an entry that I wrote for the molecularecologist.com. This one requires a little ingenuity on your part (and perhaps some craft, duct tape, construction, and/or welding skills). A nutator (AKA nutating mixer or rotating mixer) is a gently rocking/rotating platform useful…

Dr. Prepper

NOTE:  This is a repost of an entry that I wrote for the molecularecologist.com. Library preparation for next-gen sequencing has become a fact of life in many labs working with model and non-model organisms. The problems with library preparation are that (1) library prep is slow and (2) library…

Easing the pain of titration

NOTE:  This is a repost of an entry that I wrote for the molecularecologist.com. Quickly following up on Nick's post re: Illumina Library Prep, Travis Glenn pointed me towards a manuscript in Nature Protocols that proposes an alternative to the oft-grumbled-about process of titrating libraries for 454/Ion Torrent…

A penny for your method: Plate sealers

NOTE:  This is a repost of an entry that I wrote for the molecularecologist.com. You may call them plate sealers, we call them "sticky-tops" (as opposed to the rubber plate sealers we use for PCR). Whatever you call them, they seal up 96- and 384-well plates so you can…

Two sixty two eighty

We have a nanodrop in our lab. It serves us extremely well, and i really appreciate it - i was initially "broken in" on an older spectrophotometer (Beckman DU-800) that basically required you to sacrifice half your sample to get a so-so measure of DNA concentration. Because we have a…